Rapid (1 hour) high performance gel filtration chromatography resolves coexisting simple micelles, mixed micelles, and vesicles in bile.
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چکیده
منابع مشابه
Rapid (1 hour) high performance gel filtration chromatography resolves coexisting simple micelles, mixed micelles, and vesicles in bile.
We describe the use and validation of Superose 6, a high performance gel filtration medium for rapid, high resolution separation and sizing of coexisting simple micelles, mixed micelles, and vesicles in bile. We fractionated model biles (1.7-4.2 g/dl total lipid concentration, 0.15 M NaCl) composed of lecithin (L), cholesterol (Ch), and the common bile salt taurocholate (TC) using Superose 6 ge...
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High-performance thin-layer chromatography (HPTLC) is well suited to the separation of nucleobases. Most of reports on this topic are limited to the use of PEI-cellulose, ODS (octadecyl silica), and silica gel as layer materials (Randerath and Struck, 1961; Bij and Lederer, 1983; Steinberg et al., 1996). Chiral plates have also been used for separation of nucleobases, and enantiomers (Hatzack a...
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The use of aqueous mixed micellar system consisting of sodium dodecyl sulfate (SDS) and polyoxyethylene (23) dodecanol (Brij-35) as the mobile phase in reversed-phase liquid chromatography was studied. A group of chlorophenols was used as the test mixture. Adding organic modifier to the system showed that the use of low concentrations of organic additives improves efficiency in SDS/Brij-35 mixe...
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Extensive molecular dynamics (MD) simulations of binary systems of phospholipids and bile salts, a model for human bile, have been performed. Recent progress in hardware and software development allows simulation of the spontaneous aggregation of the constituents into small mixed micelles, in agreement with experimental observations. The MD simulations reveal the structure of these micelles at ...
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been characterized quite well using a variety of techniques individually and in combination over the last decade. Interest in these surfactant systems stems from a broad application base. These surfactant systems, or “detergents” have found application in oil recovery due to enhanced solubility, higher cloud points, and lower Krafft points than the corresponding pure surfactant in solution. The...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1990
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)42275-8